9J20

Structure of WDR5 in complex with KIF2A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	64.874, 47.150, 104.309
Unit cell angles	90.00, 107.47, 90.00

Refinement procedure

Resolution	34.100 - 1.850
R-factor	0.1727
Rwork	0.171
R-free	0.20630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.268
Data reduction software	autoPROC
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.950
High resolution limit [Å]	1.850	1.850
Rmerge	0.115	0.587
Rmeas	0.125	0.637
Rpim	0.048	0.244
Total number of observations	355002	51186
Number of reflections	51828	7523
<I/σ(I)>	10.2	3.4
Completeness [%]	100.0
Redundancy	6.8	6.8
CC(1/2)	0.997	0.895

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1 M HEPES (pH 7.5) and 18% PEG 600