9J1U

Structural basis of the bifunctionality of M. salinexigens ZYF650T glucosylglycerol phosphorylase in glucosylglycerol catabolism

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	111.830, 178.500, 180.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.040 - 2.720
R-factor	0.2163
Rwork	0.214
R-free	0.25390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7xdq
RMSD bond length	0.007
RMSD bond angle	1.027
Data reduction software	HKL-3000 (7.21)
Data scaling software	HKL-3000 (7.21)
Phasing software	PHASER (2.7.0)
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.110	2.790
High resolution limit [Å]	2.720	2.720
Rmerge	0.074	0.747
Rmeas	0.096	0.980
Rpim	0.051	0.626
Number of reflections	96759	6676
<I/σ(I)>	14.7
Completeness [%]	99.3
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		289	0.1 M Sodium chloride, 0.1 M BIS-TRIS propane pH 9.0, 25% Polyethylene glycol 1,500