9J1N

Mouse Spi-B Ets domain in complex with DNA containing AGAA sequence

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2022-07-14
Detector	RIGAKU RAXIS VII
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	33.290, 53.810, 100.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.310 - 2.240
R-factor	0.2163
Rwork	0.215
R-free	0.24440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.639
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.300
High resolution limit [Å]	2.240	2.240
Rmerge	0.123	1.010
Number of reflections	9111	4528
<I/σ(I)>	9.47	1.98
Completeness [%]	99.6	100
Redundancy	6.84	7.01
CC(1/2)	0.996	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293.15	0.1M MES pH5.6, 7.6% PEG8000, 8.0% Ethylene Glycol