9J1H

The binary complex structure of F2Y224-FtmOx1 mutant with alpha-ketoglutarate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	62.032, 62.077, 151.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.950 - 2.000
R-factor	0.1815
Rwork	0.172
R-free	0.19350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.941
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.950	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.118	0.470
Rmeas	0.129	0.545
Number of reflections	40043	3641
<I/σ(I)>	9.28
Completeness [%]	99.0
Redundancy	5.9
CC(1/2)	0.992	0.846

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.4	293	100 mM Bis-Tris pH 6.4 50 mM CoCl2 1.8 M ammonium sulfate