Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.216, 79.070, 87.917 |
| Unit cell angles | 90.00, 117.20, 90.00 |
Refinement procedure
| Resolution | 78.200 - 2.170 |
| R-factor | 0.21093 |
| Rwork | 0.209 |
| R-free | 0.25690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4p9w |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.599 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.200 | 2.280 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Rmerge | 0.136 | 0.862 |
| Rmeas | 0.161 | 1.058 |
| Rpim | 0.084 | 0.602 |
| Total number of observations | 184071 | 22043 |
| Number of reflections | 52760 | 7613 |
| <I/σ(I)> | 4.4 | 2.1 |
| Completeness [%] | 99.8 | |
| Redundancy | 3.5 | 2.9 |
| CC(1/2) | 0.983 | 0.369 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2M Sodium chloride, 0.1M Phosphate-citrate pH 4.2 10% (w/v) PEG 3000 |
