Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-04-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 141.430, 141.430, 80.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.100 - 3.490 |
| R-factor | 0.1979 |
| Rwork | 0.196 |
| R-free | 0.22910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4p9w |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.833 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.18.2-3874-000)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.250 | 3.580 |
| High resolution limit [Å] | 3.490 | 3.490 |
| Rmerge | 0.186 | 1.069 |
| Rmeas | 0.190 | 1.090 |
| Rpim | 0.039 | 0.212 |
| Total number of observations | 264888 | 20492 |
| Number of reflections | 10870 | 782 |
| <I/σ(I)> | 17.7 | 4.1 |
| Completeness [%] | 100.0 | |
| Redundancy | 24.4 | 26.2 |
| CC(1/2) | 0.996 | 0.880 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1M Tris-HCl pH 8.5, 2.0M Ammonium phosphate monobasic |
