Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 41 |
| Unit cell lengths | 61.379, 61.379, 233.175 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.380 - 2.430 |
| R-factor | 0.2149 |
| Rwork | 0.211 |
| R-free | 0.28220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4p9w |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.190 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.18.2-3874-000)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.380 | 2.560 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Rmerge | 0.335 | 3.095 |
| Rmeas | 0.351 | 3.245 |
| Rpim | 0.103 | 0.967 |
| Total number of observations | 352019 | 53789 |
| Number of reflections | 31076 | 4725 |
| <I/σ(I)> | 5.3 | 1.9 |
| Completeness [%] | 96.1 | |
| Redundancy | 11.3 | 11.4 |
| CC(1/2) | 0.987 | 0.447 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M MES monohydrate pH 6.5, 12 % w/v Polyethylene glycol 20,000 |
