9J1A
Structure of ConA without ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 62.239, 87.413, 88.959 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.190 - 1.480 |
| R-factor | 0.18537 |
| Rwork | 0.184 |
| R-free | 0.21318 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.730 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.190 | 1.520 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.108 | 1.434 |
| Rmeas | 0.113 | 1.548 |
| Rpim | 0.033 | 0.575 |
| Total number of observations | 21079 | |
| Number of reflections | 40754 | 2977 |
| <I/σ(I)> | 10.2 | 1.6 |
| Completeness [%] | 100.0 | |
| Redundancy | 11.4 | 7.1 |
| CC(1/2) | 0.999 | 0.741 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1.8M Ammonium Sulfate, 0.1M MES pH 6.5, 0.01M Cobalt Chloride |
