9J11

Structure of mEos3.2 in the green fluorescent state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 1 21 1
Unit cell lengths	51.357, 82.392, 61.775
Unit cell angles	90.00, 101.93, 90.00

Refinement procedure

Resolution	28.640 - 1.850
R-factor	0.1548
Rwork	0.153
R-free	0.18400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ooz
RMSD bond length	0.008
RMSD bond angle	1.462
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.730	1.950
High resolution limit [Å]	1.850	1.850
Number of reflections	43073	6286
<I/σ(I)>	12.8
Completeness [%]	100.0
Redundancy	6.8
CC(1/2)	0.999	0.842

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2 M Ammonium acetate, 0.1 M Sodium acetate trihydrate, pH 6.0, 30% (w/v) PEG 4000