9J0A

Complex structure of ANKRD11/STAG2/RAD21

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	I 1 2 1
Unit cell lengths	280.535, 48.773, 290.906
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.880 - 3.300
R-factor	0.2351
Rwork	0.233
R-free	0.27920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qnx
RMSD bond length	0.003
RMSD bond angle	0.627
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.360
High resolution limit [Å]	3.300	3.300
Rmerge	0.196	1.406
Rmeas	0.214	1.523
Rpim	0.084	0.580
Number of reflections	60932	2973
<I/σ(I)>	8	0.9
Completeness [%]	100.0	100
Redundancy	6.45	6.76
CC(1/2)	0.997	0.606

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.8	289	0.02 M citric acid, 0.08 M BIS-TRIS propane (pH 8.8), and 16% w/v polyethylene glycol 3,350