9J00

Crystal Structure Sensory Appendage Protein 2 from Anopheles culicifacies in space group P21 with three molecules per ASU

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-25
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	P 1 21 1
Unit cell lengths	58.504, 37.345, 79.437
Unit cell angles	90.00, 96.23, 90.00

Refinement procedure

Resolution	78.968 - 1.828
R-factor	0.1925
Rwork	0.190
R-free	0.23630
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.303
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	CRANK2
Refinement software	PHENIX ((1.15rc1_3423: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.968	1.860
High resolution limit [Å]	1.828	1.828
Rmeas	0.150	1.932
Number of reflections	30282	1306
<I/σ(I)>	8.5
Completeness [%]	98.9	86.8
Redundancy	5.4	3.7
CC(1/2)	0.996	0.358

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	0.1 M Lithium chloride, 0.1 M Sodium acetate pH 4.5, 30% v/v PEG 400, 0.1 M Cadmium chloride hemi(pentahydrate)