Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97923 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.130, 163.710, 87.408 |
| Unit cell angles | 90.00, 93.01, 90.00 |
Refinement procedure
| Resolution | 46.270 - 2.200 |
| R-factor | 0.1852 |
| Rwork | 0.182 |
| R-free | 0.23810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 9iku |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.439 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.270 | 2.279 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.149 | 0.922 |
| Rmeas | 0.155 | 0.958 |
| Rpim | 0.043 | 0.259 |
| Number of reflections | 54459 | 5406 |
| <I/σ(I)> | 16.69 | 3.41 |
| Completeness [%] | 97.8 | 97.26 |
| Redundancy | 13.2 | 13.5 |
| CC(1/2) | 0.998 | 0.892 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 20% v/v isopropanol, 0.1 M MES monohydrate pH 6.0, 24% w/v PEG 3350 |
