9IWB

High Resolution Crystal Structure of Glyceraldehyde-3-Phosphate Dehydrogenase from Saccharomyces cerevisiae Complexed with a Maleate Derivative

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	I 41 2 2
Unit cell lengths	116.569, 116.569, 115.635
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	82.430 - 1.950
R-factor	0.2105
Rwork	0.209
R-free	0.23680
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	DIALS
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.430	2.020
High resolution limit [Å]	1.950	1.950
Number of reflections	29278	2879
<I/σ(I)>	8.97
Completeness [%]	99.9
Redundancy	21.5
CC(1/2)	0.994	0.683

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	25% v/v PEG 3350, 0.2M Ammonium acetate, pH 6.3, 0.5M sodium chloride, 0.1M Bis-Tris