9IVY

Pseudomonas aeruginosa Histidinol dehydrogenase with NADH and Zn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER X8 PROTEUM
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-09
Detector	Bruker PHOTON III
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	67.299, 89.527, 139.871
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.560 - 2.330
R-factor	0.2022
Rwork	0.200
R-free	0.24280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.610
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.560	2.430
High resolution limit [Å]	2.330	2.330
Number of reflections	69670	3856
<I/σ(I)>	9.5
Completeness [%]	99.8
Redundancy	7.1
CC(1/2)	0.990	0.756

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.5	298	30% PEG 400,100mM NaAcetate, pH 4.5, 100mM CaAcetate