9ISP

DUF2436 domain which is frequently found in virulence proteins from Porphyromonas gingivalis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-26
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979
Spacegroup name	P 32 2 1
Unit cell lengths	101.275, 101.275, 70.168
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.240 - 2.210
R-factor	0.2388
Rwork	0.238
R-free	0.25860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.250
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.240	2.289
High resolution limit [Å]	2.210	2.210
Number of reflections	21191	2066
<I/σ(I)>	22.29
Completeness [%]	99.9
Redundancy	19.8
CC(1/2)	0.987	0.483

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.2 M Sodium Chloride, 0.1 M Tris-HCl (pH 7), 1 M sodium Citrate