9ISJ

Crystal structure of Klebsiella pneumoniae ZapA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 61 2 2
Unit cell lengths	54.173, 54.173, 330.044
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.448 - 1.800
Rwork	0.219
R-free	0.23930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.530
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.450	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	28055	2712
<I/σ(I)>	20.03
Completeness [%]	99.8
Redundancy	9
CC(1/2)	0.999	0.872

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.2 M Sodium acetate trihydrate, 0.1 M Sodium citrate pH5.5, and 10% PEG4000