Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.019, 63.097, 121.850 |
| Unit cell angles | 90.00, 91.31, 90.00 |
Refinement procedure
| Resolution | 42.110 - 2.780 |
| R-factor | 0.249 |
| Rwork | 0.244 |
| R-free | 0.32480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.126 |
| Data reduction software | HKL-3000 (7.21) |
| Data scaling software | HKL-3000 (7.21) |
| Phasing software | PHASER (2.7.0) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.110 | 2.820 |
| High resolution limit [Å] | 2.780 | 2.780 |
| Number of reflections | 66251 | 66251 |
| <I/σ(I)> | 4.2 | |
| Completeness [%] | 99.7 | |
| Redundancy | 1.34 | |
| CC(1/2) | 0.964 | 0.964 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 11% PEG4000, 0.1M Sodium Chloride, 0.1M Tris |
