9IQU
phage HY126 dCMP hydroxylase-AfhB with sustrate dCMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 65.190, 65.190, 231.933 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.840 - 1.960 |
| R-factor | 0.1985 |
| Rwork | 0.196 |
| R-free | 0.21930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.277 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.850 | 2.060 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.281 | |
| Number of reflections | 20631 | 1022 |
| <I/σ(I)> | 28.8 | |
| Completeness [%] | 98.0 | |
| Redundancy | 8 | |
| CC(1/2) | 0.999 | 0.985 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.2 M Ammonium acetate;0.1M Sodium acetate teihydrate pH4.6;15% peg 4000 |
