9IQM
CatM-L6S, a SnoaL-like protein in complex with substrate mimic L6S
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97919 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 38.617, 99.719, 58.859 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.010 - 1.700 |
| R-factor | 0.2089 |
| Rwork | 0.208 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 9iq5 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.143 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.010 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 12789 | 1178 |
| <I/σ(I)> | 13.37 | 2.38 |
| Completeness [%] | 99.0 | |
| Redundancy | 10.2 | |
| CC(1/2) | 0.997 | 0.670 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 2.0 M Ammonium sulfate, 0.1 M Sodium HEPES 7.5, 2 % v/v PEG 400 |
