9IQH
Crystal structure of sulfhydrylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-01-04 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.5405 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.732, 82.799, 77.496 |
| Unit cell angles | 90.00, 110.32, 90.00 |
Refinement procedure
| Resolution | 23.140 - 2.270 |
| R-factor | 0.165 |
| Rwork | 0.162 |
| R-free | 0.21480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.673 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.140 | 2.351 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Number of reflections | 34171 | 2285 |
| <I/σ(I)> | 17.8 | |
| Completeness [%] | 95.1 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.998 | 0.967 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 20% (w/v) polyethylene glycol 3350 and 200 mM magnesium sulfate heptahydrate at pH 5.9 |
