9IQD
Apo-MicM,homologous of AkaM, SnoaL-like protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL10U2 |
Synchrotron site | SSRF |
Beamline | BL10U2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-11-15 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97919 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.306, 72.732, 65.403 |
Unit cell angles | 90.00, 112.21, 90.00 |
Refinement procedure
Resolution | 59.540 - 1.770 |
R-factor | 0.1832 |
Rwork | 0.181 |
R-free | 0.21650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 9iq7 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.886 |
Data reduction software | XDS |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.540 | 1.830 |
High resolution limit [Å] | 1.770 | 1.770 |
Number of reflections | 53998 | 5331 |
<I/σ(I)> | 14.13 | |
Completeness [%] | 99.1 | |
Redundancy | 4.9 | |
CC(1/2) | 0.997 | 0.937 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 291.15 | 20 % w/v Polyethylene glycol 6,000, 100 mM tri-Sodium citrate; pH 5.0 |