9IQC

Monooxygenase dependent on riboflavin with dCMP and FAD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-05-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 61 2 2
Unit cell lengths	64.702, 64.702, 230.362
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	56.030 - 1.690
R-factor	0.1752
Rwork	0.174
R-free	0.19260
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.853
Data reduction software	PROCOR
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.030	1.780
High resolution limit [Å]	1.690	1.690
Rmerge	0.116
Number of reflections	32844	32844
<I/σ(I)>	25
Completeness [%]	99.8	98.8
Redundancy	12.6
CC(1/2)	1.000	0.918

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	8% v/v Tacsimate pH 6.0 20% w/v Polyethylene glycol 3350