9IQ4

Crystal structure of African swine fever virus methyltransferase EP424R in complex with S-Adenosylhomocysteine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97923
Spacegroup name	P 1 21 1
Unit cell lengths	79.113, 56.918, 100.465
Unit cell angles	90.00, 112.30, 90.00

Refinement procedure

Resolution	48.540 - 2.420
R-factor	0.205
Rwork	0.205
R-free	0.21230
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.105
Data reduction software	autoPX
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.420	2.420
Number of reflections	31642	2304
<I/σ(I)>	14.06
Completeness [%]	99.6
Redundancy	6.63
CC(1/2)	0.640	0.863

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	19%(w/v) PEG 3350, 200 mM Ammonium citrate dibasic