9IP6

The complex of rice immune receptor RGA5-HMA8 with rice blast effector protein AVR1-CO39

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	40.600, 126.814, 47.231
Unit cell angles	90.00, 110.74, 90.00

Refinement procedure

Resolution	35.720 - 2.630
R-factor	0.1921
Rwork	0.185
R-free	0.25650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.902
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.680
High resolution limit [Å]	2.630	2.630
Number of reflections	13262	654
<I/σ(I)>	13.83
Completeness [%]	99.8
Redundancy	6
CC(1/2)	0.997	0.892

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		300	0.1 M Sodium citrate tribasic dihydrate pH 5.5, 20% w/v Polyethylene glycol 3,000