9INT

Crystal structure of the complex of the beta,kappa-carrageenase Cgbk16A from Wenyingzhuangia fucanilytica with an oligosaccharide of furcellaran

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	47.770, 76.930, 99.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.580 - 1.560
R-factor	0.1588
Rwork	0.158
R-free	0.17710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.023
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.580	1.620
High resolution limit [Å]	1.560	1.560
Rmerge	0.049
Rmeas	0.053
Rpim	0.020
Number of reflections	52972	51770
<I/σ(I)>	27.9	5.5
Completeness [%]	97.9	93.74
Redundancy	12.7
CC(1/2)	1.000	0.963

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	25.5% PEG 4000, 15% Glycerol, 0.17 M Ammonium sulfate