9INK
Crystal structure of beta-carotene-binding protein (BBP) from Schistocerca gregaria complexed with beta-carotene
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17UM |
| Synchrotron site | SSRF |
| Beamline | BL17UM |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-24 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 55.390, 75.510, 224.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.880 - 2.700 |
| R-factor | 0.22412 |
| Rwork | 0.221 |
| R-free | 0.27766 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.313 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.880 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.411 | 2.850 |
| Rmeas | 0.446 | 3.089 |
| Rpim | 0.171 | 1.178 |
| Total number of observations | 89039 | 11717 |
| Number of reflections | 13341 | 1729 |
| <I/σ(I)> | 4.4 | 0.7 |
| Completeness [%] | 99.6 | |
| Redundancy | 6.7 | 6.8 |
| CC(1/2) | 0.981 | 0.310 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 288 | 0.1 M Potassium acetate, pH 4.0, 0.2 M Potassium chloride, 20% PEG 6000 |
