9INC
High resolutional Crystal Structure of human H2A.Z-H2B dimer in complex with human YL1-Z domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-04-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 116.093, 126.990, 70.757 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.770 - 2.010 |
| R-factor | 0.2214 |
| Rwork | 0.220 |
| R-free | 0.25730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.762 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHENIX (1.20.1_4487_000) |
| Refinement software | PHENIX ((1.20.1_4487_000)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.680 | 2.060 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.102 | 1.574 |
| Number of reflections | 35203 | 2574 |
| <I/σ(I)> | 13.1 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.9 | 9 |
| CC(1/2) | 0.999 | 0.593 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 45% PEG200 0.1 M HEPES pH 7.5 |
