9IN9

Structure of the viral channelrhodopsin OLPVR1 at low pH obtained by soaking

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-06
Detector	DECTRIS EIGER2 X CdTe 16M
Wavelength(s)	0.8856
Spacegroup name	P 21 21 2
Unit cell lengths	46.235, 115.755, 53.214
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.420 - 1.410
R-factor	0.1892
Rwork	0.188
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7aky
RMSD bond length	0.008
RMSD bond angle	0.931
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.420	1.550
High resolution limit [Å]	1.410	1.410
Rpim	0.025	0.952
Number of reflections	36461	1824
<I/σ(I)>	13.1	0.8
Completeness [%]	92.8	75.9
Redundancy	4.9	6.6
CC(1/2)	0.999	0.316

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	8	293	100 mM Tris, 900 mM NaCl, and 24% (w/v) PEG 6000; Soaked: 100 mM sodium citrate pH 2.5, 900 mM NaCl, and 24% (w/v) PEG 6000