9IN7
True-atomic resolution crystal structure of the closed state of the viral channelrhodopsin OLPVR1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-03 |
| Detector | DECTRIS EIGER2 X CdTe 16M |
| Wavelength(s) | 0.8856 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 46.498, 114.948, 53.171 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.480 - 1.130 |
| R-factor | 0.153 |
| Rwork | 0.151 |
| R-free | 0.18380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7aky |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.062 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.480 | 1.230 |
| High resolution limit [Å] | 1.130 | 1.130 |
| Rpim | 0.022 | 1.066 |
| Number of reflections | 78116 | 3905 |
| <I/σ(I)> | 12.1 | 0.7 |
| Completeness [%] | 94.3 | 70.8 |
| Redundancy | 4.8 | 5.3 |
| CC(1/2) | 0.999 | 0.220 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 8 | 293 | 100 mM Tris, 900 mM NaCl, and 24% (w/v) PEG 6000 |
