9IMX
Immune complex of HEV E2s and P1-5B nanobody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-05 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 51.393, 51.402, 265.172 |
| Unit cell angles | 90.00, 92.74, 90.00 |
Refinement procedure
| Resolution | 66.220 - 2.200 |
| R-factor | 0.27511 |
| Rwork | 0.217 |
| R-free | 0.24520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ggq 7kn5 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.640 |
| Data reduction software | AutoProcess |
| Data scaling software | AutoProcess |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.220 | 2.310 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmeas | 0.314 | |
| Rpim | 0.120 | |
| Number of reflections | 70266 | 66929 |
| <I/σ(I)> | 4.9 | |
| Completeness [%] | 98.6 | 97.8 |
| Redundancy | 6.9 | |
| CC(1/2) | 0.929 | 0.379 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | 6% isopropanol, 0.1M sodium acetate pH 4.5, 20% PEG MME550 |
