9IMW
Crystal structure of N-terminal domain of human Hsp90
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-01-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 65.849, 89.309, 99.998 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.000 - 1.527 |
| R-factor | 0.1636 |
| Rwork | 0.163 |
| R-free | 0.18110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.716 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.000 | 1.610 |
| High resolution limit [Å] | 1.527 | 1.530 |
| Rmerge | 0.133 | 2.137 |
| Rmeas | 0.141 | 2.301 |
| Rpim | 0.046 | 0.837 |
| Total number of observations | 47003 | |
| Number of reflections | 45027 | 6487 |
| <I/σ(I)> | 12.5 | 1.5 |
| Completeness [%] | 100.0 | |
| Redundancy | 9.4 | 7.2 |
| CC(1/2) | 0.998 | 0.461 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20-25% (w/v) PEG 4000, 200mM magnesium chloride, 100mM Tris-HCl, pH 6.5 |
