9IMU

The complex of rice immune receptor RGA5-HMA8 with rice blast effector protein AVR-PikD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 61
Unit cell lengths	66.410, 66.410, 84.446
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.040 - 2.380
R-factor	0.1998
Rwork	0.196
R-free	0.23780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5a6w
RMSD bond length	0.009
RMSD bond angle	1.299
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.420
High resolution limit [Å]	2.380	2.380
Number of reflections	8536	410
<I/σ(I)>	45.2
Completeness [%]	99.8
Redundancy	19.3
CC(1/2)	0.986	0.951

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		300	0.1 M Succinic acid pH 7.0, 15% w/v Polyethylene glycol 3,350