Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978520 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 105.270, 158.140, 179.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.350 - 2.750 |
| R-factor | 0.212 |
| Rwork | 0.211 |
| R-free | 0.25510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.018 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.960 | 2.820 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Number of reflections | 78694 | 5756 |
| <I/σ(I)> | 17.8 | 1.6 |
| Completeness [%] | 99.9 | |
| Redundancy | 13.2 | |
| CC(1/2) | 0.999 | 0.671 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 6% PEG 4000, 5% glycerol, 0.1M MES, 30mM CaCl2, 30mM MgCl2, pH 6.7 |
