9IMJ

Bacteriophage T6 topoisomerase II ATPase domain crystal strcuture

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-X
Temperature [K]	100
Detector technology	CCD
Collection date	2024-06-27
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	90.989, 90.989, 102.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.790 - 2.800
R-factor	0.236
Rwork	0.233
R-free	0.28950
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (v1.20)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.750
High resolution limit [Å]	2.700	7.320	2.700
Rmerge	0.101	0.047	0.736
Rmeas	0.104	0.049	0.760
Rpim	0.026	0.013	0.189
Total number of observations	225456
Number of reflections	13967	773	685
<I/σ(I)>	12.1
Completeness [%]	100.0	99.2	100
Redundancy	16.1	14	15.8
CC(1/2)	0.998	0.998	0.972

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289	PEG3350, succinic acid