9ILT

Crystal structure of alternative complex III from Chloroflexus aurantiacus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	142.016, 153.063, 173.381
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.300 - 3.250
R-factor	0.2507
Rwork	0.249
R-free	0.28580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8k9f
RMSD bond length	0.003
RMSD bond angle	0.921
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.300	3.366
High resolution limit [Å]	3.250	3.250
Number of reflections	57488	5927
<I/σ(I)>	10.44
Completeness [%]	89.8
Redundancy	6.7
CC(1/2)	0.996	0.748

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	100 mM glycine at pH 9.0 and 26% (w/v) PEG 400