9ILD
Complex structure of Cap3 and CD-NTase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-06-07 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.987 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.051, 54.843, 59.606 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.206 - 2.179 |
R-factor | 0.2333 |
Rwork | 0.228 |
R-free | 0.28260 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.477 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.16_3549: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.206 | 2.300 |
High resolution limit [Å] | 2.179 | 2.179 |
Rmerge | 0.117 | 0.839 |
Number of reflections | 9260 | 1210 |
<I/σ(I)> | 13.1 | |
Completeness [%] | 98.5 | |
Redundancy | 13.1 | |
CC(1/2) | 0.997 | 0.690 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2 M NH4Ac, 0.1 M NaAc (pH 4.6) and 30% PEG 4000 |