9IKB

Crystal structure of heterotrimeric Kinesin-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 43 21 2
Unit cell lengths	107.445, 107.445, 255.397
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.570 - 3.540
R-factor	0.2317
Rwork	0.230
R-free	0.27050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.512
Data reduction software	HKL-2000 (v1.0)
Data scaling software	HKL-2000 (v1.0)
Phasing software	PHASER (v2.8.3)
Refinement software	PHENIX ((1.21.1_5286: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.600	3.730
High resolution limit [Å]	3.540	3.540
Number of reflections	17828	1558
<I/σ(I)>	15.3	2.3
Completeness [%]	93.8	57.7
Redundancy	13.3	12.6
CC(1/2)	0.999	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	22%(w/v) PEG3350, 0.2M Sodium malonate