9IK0

Crystal structure of PrfaH encoded by IncX3 plasmids

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97856
Spacegroup name	P 43
Unit cell lengths	57.695, 57.695, 116.465
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.210 - 2.000
R-factor	0.1808
Rwork	0.179
R-free	0.21420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AlphaFold
RMSD bond length	0.002
RMSD bond angle	0.534
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.210	2.072
High resolution limit [Å]	2.000	2.000
Rmerge	0.064	0.593
Number of reflections	25086	2361
<I/σ(I)>	23.4
Completeness [%]	97.6	92.66
Redundancy	9.8
CC(1/2)	0.999	0.903

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	291	0.1 M Sodium citrate tribasic dihydrate pH 4.8, 15-18% w/v PEG20,000