9IJX
Crystal structure of the mouse Spef1 coiled-coil domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.949 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 60.150, 104.360, 72.270 |
| Unit cell angles | 90.00, 90.04, 90.00 |
Refinement procedure
| Resolution | 26.090 - 2.450 |
| R-factor | 0.19707 |
| Rwork | 0.195 |
| R-free | 0.23436 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.312 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.510 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.060 | 0.493 |
| Rpim | 0.049 | 0.415 |
| Number of reflections | 15881 | 1167 |
| <I/σ(I)> | 11.4 | 2.4 |
| Completeness [%] | 96.6 | 93.7 |
| Redundancy | 4.4 | |
| CC(1/2) | 0.996 | 0.609 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.1M BICINE, 3%(w/v) Dextran sulfate sodium, 15% (w/v) PEG20000. |
