9IIS

GDP-fucose pyrophosphorylase part of FKP with a SUMO tag

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-09
Detector	RAYONIX MX300HE
Wavelength(s)	1.0000
Spacegroup name	I 2 2 2
Unit cell lengths	86.585, 109.854, 144.107
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.120 - 2.360
R-factor	0.2052
Rwork	0.203
R-free	0.25390
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.649
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.430
High resolution limit [Å]	2.350	2.350
Rmerge	0.065	0.771
Number of reflections	28528	2831
<I/σ(I)>	23.4	2.1
Completeness [%]	99.4	99.3
Redundancy	4.9	4.8
CC(1/2)	0.970	0.893

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	PEG 3350, ammonium acetate, HEPES