9IIE
Cryogenic Temperature Crystal Structure of Fc Fragment of Human IgG1 from Biosimilar VEGF-Trap
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU PhotonJet-R |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2024-06-03 |
Detector | RIGAKU HyPix-Arc 150 |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.920, 80.480, 137.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.840 - 3.140 |
R-factor | 0.2621 |
Rwork | 0.257 |
R-free | 0.30860 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.503 |
Data reduction software | CrysalisPro |
Data scaling software | CrysalisPro |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 137.080 | 3.220 |
High resolution limit [Å] | 3.140 | 3.140 |
Number of reflections | 9844 | 683 |
<I/σ(I)> | 2.26 | |
Completeness [%] | 96.5 | 98.6 |
Redundancy | 3.6 | 3.9 |
CC(1/2) | 0.748 | 0.155 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1M Bis-Tris pH 6.5, 25% w/v Polyethylene glycol 3350 |