9IIC
Crystal structure of HOIP RING2-LDD in complex with STK4 KD domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-11-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 138.989, 96.363, 115.773 |
| Unit cell angles | 90.00, 118.82, 90.00 |
Refinement procedure
| Resolution | 68.870 - 2.780 |
| R-factor | 0.2392 |
| Rwork | 0.237 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.673 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.570 | 2.830 |
| High resolution limit [Å] | 2.780 | 2.780 |
| Rmerge | 0.080 | 0.789 |
| Number of reflections | 32995 | 1682 |
| <I/σ(I)> | 13.7 | 2.2 |
| Completeness [%] | 97.5 | 98.4 |
| Redundancy | 6.6 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2 M Ammonium citrate tribasic (pH 7.0), 20% w/v Polyethylene glycol 3350 |
