9IHA
A-SPECTRIN SH3 DOMAIN V9L, A11V, M25V, V46F, V58L MUTANT
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2022-03-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.021, 36.562, 64.926 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.463 - 1.013 |
| Rwork | 0.163 |
| R-free | 0.17400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.033 |
| RMSD bond angle | 3.008 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.463 | 1.030 |
| High resolution limit [Å] | 1.013 | 1.013 |
| Rmerge | 0.059 | 1.689 |
| Rpim | 0.018 | 0.510 |
| Number of reflections | 40408 | 1981 |
| <I/σ(I)> | 21.7 | 1.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.4 | |
| CC(1/2) | 0.998 | 0.649 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 2M Ammonium Sulfate and 0.1M Bis-Tris |
