9IGY
Structure of a Chimeric Protein Composed of the SNX5 PhoX Domain and the N-terminal domain of NCOA7-AS,crystal form II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-22 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.900, 76.990, 110.850 |
| Unit cell angles | 90.00, 100.18, 90.00 |
Refinement procedure
| Resolution | 46.160 - 2.300 |
| R-factor | 0.2294 |
| Rwork | 0.227 |
| R-free | 0.26210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.404 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21rc1_5008) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.160 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmeas | 0.162 | 1.301 |
| Number of reflections | 33878 | 4141 |
| <I/σ(I)> | 10.52 | 1.64 |
| Completeness [%] | 97.6 | 99.9 |
| Redundancy | 6.79 | 7.01 |
| CC(1/2) | 0.990 | 0.695 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.1 M Sodium citrate tribasic dihydrate pH 5.0, 30% v/v Jeffamine ED-2001 pH 7.0 |
