9IFU
FSP1 (tetrapod ancestor) bound to FAD and NAD+ and compound 1
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-07-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.965459 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.567, 79.684, 113.062 |
| Unit cell angles | 90.00, 98.61, 90.00 |
Refinement procedure
| Resolution | 64.970 - 1.550 |
| R-factor | 0.18222 |
| Rwork | 0.179 |
| R-free | 0.24249 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.341 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.970 | 1.842 |
| High resolution limit [Å] | 1.550 | 1.551 |
| Rmerge | 0.106 | 0.393 |
| Rmeas | 0.133 | 0.484 |
| Rpim | 0.079 | 0.279 |
| Total number of observations | 6050 | |
| Number of reflections | 46979 | 2349 |
| <I/σ(I)> | 5.1 | 2.2 |
| Completeness [%] | 83.7 | |
| Redundancy | 2.5 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.06 M MgCl2, CaCl2, 0.1M imidazole/MES monohydrate (acid) pH 6.5, 12.5% MPD, 12.5% PEG1000, 12.5% PEG3350 |
