9IFT
FSP1 (tetrapod ancestor) bound to FAD and NAD+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-19 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.873128 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.947, 80.528, 60.918 |
| Unit cell angles | 90.00, 100.69, 90.00 |
Refinement procedure
| Resolution | 48.090 - 1.450 |
| R-factor | 0.17656 |
| Rwork | 0.176 |
| R-free | 0.20350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.663 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.090 | 1.470 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.096 | 1.995 |
| Rmeas | 0.104 | 2.164 |
| Rpim | 0.038 | 0.822 |
| Total number of observations | 24050 | |
| Number of reflections | 72942 | 3580 |
| <I/σ(I)> | 10 | 1 |
| Completeness [%] | 99.0 | |
| Redundancy | 6.9 | 6.7 |
| CC(1/2) | 0.999 | 0.455 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.06 M MgCl2, 0.06 CaCl2, 0.1 M HEPES/MOPS (acid) pH 7.5, 12.5% PEG1000, 12.5% PEG3350, 12.5% MPD |
