9IF2
ZnT1 CTD regulation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-19 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.538, 83.062, 85.862 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.699 - 2.110 |
| Rwork | 0.225 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.858 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430 (refmacat 0.4.88)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.700 | 2.170 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rmerge | 0.070 | 5.158 |
| Number of reflections | 32655 | 2629 |
| <I/σ(I)> | 17.5 | 0.5 |
| Completeness [%] | 100.0 | |
| Redundancy | 13.4 | |
| CC(1/2) | 1.000 | 0.225 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1M sodium acetate pH 4.6, 0.14M calcium acetate, 15% w/v% PEG 3000 |
