9ICM

DNA POLYMERASE BETA (POL B) (E.C.2.7.7.7) COMPLEXED WITH SIX BASE PAIRS OF DOUBLE STRANDED DNA (NO 5'-PHOSPHATE)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1995-04-09
Detector	SDMS
Spacegroup name	P 21 21 2
Unit cell lengths	178.698, 57.867, 48.556
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.900
R-factor	0.155
Structure solution method	DIFFERENCE FOURIER
Starting model (for MR)	9icw
RMSD bond length	0.018
RMSD bond angle	2.800
Data reduction software	SDMS
Data scaling software	SDMS
Phasing software	TNT (V. 5-D)
Refinement software	TNT (5-D)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.000
High resolution limit [Å]	2.900	2.900
Number of reflections	10507
Completeness [%]	89.0	82
Redundancy	1.8	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		THIS ENTRY DESCRIBES THE SAME CRYSTAL STRUCTURE REPORTED IN ENTRY 9ICW, WITH THE EXCEPTION THAT THE DNA STRAND DESIGNATED AS THE PRIMER IS NOT 5'-PHOSPHORYLATED. SEE REFERENCE 1 FOR DETAILS CONCERNING EXPERIMENTAL PROCEDURES, RESULTS, AND DISCUSSIONS FOR THIS STRUCTURE., pH 6.50