9ICE
DNA POLYMERASE BETA (POL B) (E.C.2.7.7.7) COMPLEXED WITH SEVEN BASE PAIRS OF DNA; SOAKED IN THE PRESENCE OF DATP (1 MILLIMOLAR) AND CUCL2 (0.1 MILLIMOLAR)
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | AREA DETECTOR |
Collection date | 1995-04-22 |
Detector | SDMS |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 178.839, 57.871, 48.625 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.300 |
R-factor | 0.186 |
Structure solution method | DIFFERENCE FOURIER |
Starting model (for MR) | 8icb |
RMSD bond length | 0.016 |
RMSD bond angle | 2.900 |
Data reduction software | SDMS |
Data scaling software | SDMS |
Phasing software | TNT (V. 5-D) |
Refinement software | TNT (5-D) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.400 |
High resolution limit [Å] | 3.300 | 3.300 |
Number of reflections | 7578 | |
Completeness [%] | 90.0 | 94 |
Redundancy | 2 | 1.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | THIS ENTRY DESCRIBES THE STRUCTURE THAT RESULTED WHEN A COCRYSTAL OF HUMAN DNA POLYMERASE BETA COMPLEXED WITH 7 BASE PAIRS OF DNA (SEE ENTRY 9ICJ AND REFERENCE 1) HAD BEEN SOAKED IN THE FOLLOWING SOLUTION FOR 24 HOURS: PEG 3350, 16% MES, 100 MILLIMOLAR, PH 6.5 NACL, 150 MILLIMOLAR DATP, 1 MILLIMOLAR CUCL2, 0.1 MILLIMOLAR SEE REFERENCE 2 FOR DETAILS CONCERNING EXPERIMENTAL PROCEDURES, RESULTS, AND DISCUSSION FOR THIS STRUCTURE. |