9ICD
CATALYTIC MECHANISM OF NADP+-DEPENDENT ISOCITRATE DEHYDROGENASE: IMPLICATIONS FROM THE STRUCTURES OF MAGNESIUM-ISOCITRATE AND NADP+ COMPLEXES
Experimental procedure
Spacegroup name | P 43 21 2 |
Unit cell lengths | 105.100, 105.100, 150.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 5.000 * - 2.500 |
R-factor | 0.181 * |
Rwork | 0.181 |
RMSD bond length | 0.016 |
RMSD bond angle | 3.060 |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.500 * |
Rmerge | 0.115 * |
Total number of observations | 141100 * |
Number of reflections | 29217 * |
Completeness [%] | 96.5 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 5.4 * | taken from Hurley, J.H. et al (1989). Proc. Natl. Acad. Sci. USA, 86, 8635-8639. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 28 (mg/ml) | |
2 | 1 | 1 | ammonium sulfate | 34 (%sat) | |
3 | 1 | 1 | 100 (mM) | ||
4 | 1 | 1 | 35 (mM) | ||
5 | 1 | 1 | citric acid | 9 (mM) | |
6 | 1 | 1 | dithiothreitol | 0.2 (mM) |